Innovation Showcase
In this talk, we’ll discuss new genetic stability data on cultures maintained in eTeSR™, a novel human pluripotent stem cell (hPSC) maintenance medium, specifically developed to support routine single-cell passaging. The study comprises over 250 SNP microarray samples collected from clones derived using three hPSC lines and maintained for 20 weeks in four media conditions. The study shows that clones maintained in eTeSR™ acquire fewer de novo cytogenetic changes compared to two alternative, commercially available maintenance media. This improvement in genetic stability can be attributed to the absence of 20q11 gains in eTeSR™-maintained cultures, a recurrent abnormality that has been well-documented in hPSC cultures. This study provides a better understanding of culture-acquired genetic aberrations in hPSCs and demonstrates that innovative media formulations such as eTeSR™ can address major challenges in the hPSC field.