Exhibit Hall Theater
Human induced pluripotent stem cells (hiPSCs) are pivotal in advancing tissue engineering, particularly for transplantation therapy and disease modeling. A sustainable approach to organ-scale tissue engineering demands the production of billions of human cells for bioprinting, especially wholly cellular bioinks. However, scalability challenges in conventional 2D cell culture methods, such as cost, space, and handling constraints, present significant hurdles. To address these issues, we optimized and developed a robust, scalable pipeline to produce hiPSC aggregates (hAs) at 1L scale using an automated stirred-tank bioreactor system for bioprinting applications. These pluripotent aggregates were subsequently differentiated into derivatives of the three germ layers, including cardiac, vascular, cortical, and intestinal organoids. The aggregates were successfully compacted into wholly cellular bioinks for rheological analysis and 3D bioprinting. The 3D bioprinted tissues exhibited high post-printing viability and the potential for vascular and neuronal differentiation, highlighting a promising pathway for billion cell-scale organ engineering. Our next objective is to further expand this process by implementing a 10L scale bioreactor system to expand and differentiate the cells to enhance cell yield and process efficiency.