Exhibit Hall Theater

INNOVATIVE scFAST-seq TECHNOLOGY: ACCESSING ANALYSIS FROM MUTATION, REGULATION TO EXPRESSION

Single-cell RNA sequencing (scRNA-seq) is pivotal for exploring cellular heterogeneity, but current methods have limitations. They rely on oligo-dT primers, leading to poor detection of non-polyadenylated transcripts and bias towards RNA ends. To overcome these challenges, we introduce Single Cell Full-length RNA Sequence Transcriptome-seq (scFAST-seq), an innovative, rapid, and cost-effective method. scFAST-seq combines semi-random priming, efficient reverse transcription, and rRNA removal, yielding full-length RNA libraries for up to 12,000 cells in 8 hours. It excels in detecting non-polyadenylated transcripts, covers longer transcript lengths, and identifies more splice junctions. scFAST-seq also shines in non-coding transcript discovery. When coupled with targeted region enrichment, it can detect somatic mutations and tumor cell status. In summary, scFAST-seq outperforms 3’ scRNA-seq, offering sensitivity, affordability, and enhanced capabilities crucial for studying cellular heterogeneity and advancing precision medicine.